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. 2016 Mar 9;7:10940. doi: 10.1038/ncomms10940

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(a) Multi-photon microscope images from peptide 8 (5 μM) (A), RFP-expressing A. fumigatus (B), second harmonic generation from collagen fibres (C) and merged (D) in ex vivo human lung tissue. Scale bar, 10 μm. (b) (A) Fluorescence lifetime image of 8-stained A. fumigatus in ex vivo human lung tissue. White arrows point autofluorescent tissue structures and yellow arrows point 8-stained fungal cells. (B) Corresponding fluorescence image of 8-stained A. fumigatus (green) and collagen fibres (second harmonic generation, cyan) for the fluorescence lifetime image in A. Scale bar, 20 μm.