Figure 3. CSR in a panel of human B-cell lymphoma lines.

Ten different human B-cell lymphoma lines (JEKO-1, GRANTA-519, UPN-1, UPN-2, MAVER-1, MINO, Z-138, BL-41, BJAB and MEC-1) were transduced with lentiviruses expressing Cas9 nuclease and gRNAs targeting Sμ and Sγ3, Sγ1, or Sα1 flanking regions. Two days later, cell lines were selected with puromycin (0.2 μg ml⁻¹) for 3 days. Live cells were collected, co-stained with CD19 and IgG or IgA antibodies, and analysed by flow cytometry. As a control, non-transduced or single lentivirus-transduced cells were used. Data were analysed by FlowJo software. Mean±s.d.; n=3 biological replicates for each condition.