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. 2016 Mar 9;7:10939. doi: 10.1038/ncomms10939

Figure 4. FAM21 regulates a precise localization of SNX27 at an endosomal subdomain through their interaction.

Figure 4

(a) hTERT-RPE1 cells transfected with the indicated SNX27 or control (siCTL) siRNAs were immunostained for SNX27 (green) and GM130 (red). Insets are magnified views of the Golgi area. Merged images with 4',6-diamidino-2-phenylindole staining (blue) are to the right. (b) To measure the size of each punctum, 30 cells of each group were analysed by ImageJ using SNX27 signals from a. (c) hTERT-RPE1 cells transfected with the indicated siRNAs were immunoblotted. (d) The levels of SNX27 mRNA in hTERT-RPE1 cells transfected with the indicated siRNAs were determined by quantitative real-time PCR. (e,f) hTERT-RPE1 cells stably expressing FLAG-SNX27, WT or ΔFERM mutant were immunostained for FLAG epitope (green) along with EEA1 (e, red) or FAM21 (f, red). Merged images with 4',6-diamidino-2-phenylindole (blue) staining and magnified views of insets are to the right. (g) To measure the total size of puncta visualized by FLAG signals from e, 30 cells of each group were analysed with ImageJ. Graphs express means±s.d. **P<0.01; NS, not significant. Scale bars, 10 μm.