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. 2016 Mar 9;7:10939. doi: 10.1038/ncomms10939

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(a) hTERT-RPE1 cells transfected with the indicated specific or control (siCTL) siRNAs were immunostained for GLUT1 (green) and GM130 (red). (b) Co-localization between GLUT1 and GM130 was analysed by calculation of Pearson's coefficient. Graphs express means±s.d. (n=3; >30 cells per group). (c) hTERT-RPE1 cells transfected with indicated siRNAs were immunostained for PI4KB (green) and GM130 (red). (d) Intensities of PI4KB at the Golgi were measured from 30 cells per group from c. Total intensities of PI4KB were divided by the Golgi area. To calculate a fold-increase from control cells, averages of experimental groups were divided by those of control cells. Graphs express means±s.d. (e) hTERT-RPE1 cells transfected with the indicated siRNAs were immunoblotted. (f–h) hTERT-RPE1 cells transfected with the indicated siRNAs were immunostained as shown. Insets are magnified views of the Golgi area in g. Merged images with 4',6-diamidino-2-phenylindole staining are to the right. Scale bars, 10 μm. *P<0.05, **P<0.01, ****P<0.0001; NS, not significant.