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. 2016 Mar 9;7:10939. doi: 10.1038/ncomms10939

Figure 7. Model for roles of the SNX27–retromer–WASH complex in cargo sorting.

Figure 7

After internalization, cargoes harbouring a PDZ-binding motif such as β2AR and GLUT1 bind to a PDZ domain of SNX27. SNX27 serves as an adaptor linking its cargoes to the endosomal tubules through its interaction. Interaction of its PDZ domain with VPS26 of retromer prevents lysosomal entry of cargoes, whereas the interaction of its FERM domain with FAM21 of the WASH complex prevents its transport to the Golgi apparatus. Therefore, these interactions within the SNX27–retromer–WASH complex direct the cargoes towards recycling to the plasma membrane. The WASH complex, in particular, functions in localizing SNX27 at a specialized endosomal subdomain. In addition, FAM21 controls PI4KB levels at the Golgi, resulting in maintaining proper levels of PI(4)P at the Golgi, which prevents dissociation of cargoes from the complex at the Golgi.