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. 2016 Jan 5;24(3):564–569. doi: 10.1038/mt.2015.192

Figure 1.

Figure 1

CRISPR-mediated deletion of a large region in mouse Dmd gene in vitro. (a) Diagram showing the genomic locus of mouse X-chromosome and the gRNA targeting sites. The mutant exon 23 is highlighted in yellow. (b) Polymerase chain reaction (PCR) analysis of genomic DNA extracted from C2C12 cells treated with or without gRNA and Cas9 constructs. (c) RT-PCR analysis of the dystrophin transcript expression in C2C12 cells. (d) PCR analysis of genomic DNA extracted from mdx myoblasts transduced with or without gRNA (Ad-i20/i23) and Cas9 expressing adenovirus. (e) RT-PCR analysis of the dystrophin transcript expression in mdx myoblasts as treated in (d). (f) DNA sequencing analysis of the smaller RT-PCR product (475 bp) in (c) and (e). Arrows indicate the expected bands after gene editing. All data are representative of a minimum of three experiments.