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. 2016 Jan 5;24(3):564–569. doi: 10.1038/mt.2015.192

Figure 2.

Figure 2

Osmotic shock-induced calcium sparks in mdx muscle fibers treated with or without mCherry-2A-Cas9/gRNA. (a) RT-PCR analysis of the total RNA extracted from the control flexor digitorum longus (FDB) muscles (Ctrl) or those electroporated with mCherry-2A-Cas9/gRNA plasmids (EP). (b) Osmotic shock-induced calcium sparks at ~3 minutes and ~10 minutes in isolated FDB muscle fibers from mdx mice electroporated with (left) or without (right) mCherry-2A-Cas9/gRNA plasmids. Arrows point to the calcium sparks located at the center of the muscle fibers. Scale bar: 50 µm. (c) Statistical analysis of calcium sparks in mdx muscle fibers electroporated with (male mdx/EP, n = 3) or without (male mdx, n = 4) mCherry-2A-Cas9/gRNA plasmids immediately (Early) or 10 minutes after osmotic shock and male WT controls (n = 4). *P < 0.05.