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. 2016 Jan 5;24(3):564–569. doi: 10.1038/mt.2015.192

Figure 4.

Figure 4

Functional rescue of dystrophin in mdx mice by CRISPR-mediated gene surgery. (a,b) Evans blue dye (EBD) uptake in the gastrocnemius muscles from male WT (n = 4), mdx (n = 4) and mdx (n = 4) mice injected with cas9/gRNA adenovirus at rest (a) or after downhill treadmill running exercise (b). The images are representative of four experiments. Red: EBD, green: GFP, blue: 4′,6-diamidino-2-phenylindole (DAPI). Scale bar: 100 µm. (c,d) Statistical analysis of EBD-positive muscle fiber percentage in WT, mdx and mdx/Ad preparations at rest (c) and after downhill treadmill running exercise (d). *P < 0.05; ***P < 0.001.