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. 2015 Dec 1;24(3):527–535. doi: 10.1038/mt.2015.200

Figure 4.

Figure 4

Targeting a transcriptional effector to the DUX4 promoter or exon 1 has no effect on expression of several off-target genes. Levels of Jumonji or KLF14 and UBR4 were assessed by quantitative reverse transcriptase polymerase chain reaction in mock-infected cultures or in cultures expressing the SunTag activator system alone or with sgRNA #3 or #6 (as in Figure 1b, lanes 1, 2, 5, and 8). Jumonji contains an off-target match (12-bp seed + PAM) to sgRNA #3 in intron 7. KLF14 and UBR4 lie 28 and 76 kb downstream of off-target matches (9 bp seed + PAM) to sgRNA #6. Refer to text for more details. Data are plotted as the mean + SD value of at least three independent experiments, with relative mRNA expression for the mock-infected cells set to 1.