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. 2016 Jan 12;24(3):636–644. doi: 10.1038/mt.2015.218

Figure 2.

Figure 2

Comparison of RGN cleavage activities at endogenous human loci. The cleavage activity of the six RGNs was assessed at two human loci, PRKDC (a) and CARD11 (b). HEK293T cells were transfected with the indicated RGNs and genomic DNA was extracted 3 days later. Upon PCR amplification of the target regions, the frequency of target disruption was assessed with the mismatch-sensitive T7EI assay. Catalytically inactive Cas9 (dCas9) variants were used as controls. Arrows indicate the positions of the expected cleavage products. Numbers at the bottom show the average percentage of modified alleles (n = 3). RGN, RNA-guided nuclease.