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. 2016 Jan 26;24(3):536–547. doi: 10.1038/mt.2015.226

Figure 2.

Figure 2

Re-expression of candidate TSGs (C13ORF18, CCNA1, TFPI2 and Maspin) in tumor-derived cell lines. (a) Schematic representations of 18-bp ZFPs (not on scale) binding to DNA. Also shown are the promoter region of CCNA1, TFPI2, Maspin and C13ORF18 stretching from −750 to +750 bp relative to the main TSS of the corresponding genes. Indicated are the targeted sites of the ZFPs directed to the CCNA1, TFPI2, Maspin and C13ORF18 promoter, as well as ZFPs which successfully demonstrated gene expression modulation when fused to effector domains. CpGs are indicated as vertical bars. Gene expression levels of C13ORF18 (b), CCNA1 (c), TFPI2 (d) and Maspin (e) mRNA relative to empty vector after retroviral transduction with gene-targeting ATFs (C13ORF18: 3ab-VP64, 5ab-VP64; CCNA1: 12ab-VP64; TFPI2: 43ab-VP64; and Maspin: 126-VP64) in CSCC-7, CSCC-8, CC-10 and CC-11. CC-11 cells were also transduced with cDNA overexpression constructs of C13ORF18, CCNA1, TFPI2 and Maspin. Each bar represents the mean of in general three independent measured in triplicate ± SEM. Quantification of mRNA was performed using qRT-PCR and induction levels were normalized to GAPDH and relative to an empty vector.