Skip to main content
. 2016 Jan 26;24(3):536–547. doi: 10.1038/mt.2015.226

Figure 6.

Figure 6

TET2-mediated reduction of cell growth by targeting TFPI2. (a) Visualization of CC-11 colonies after expressing the TFPI2-targeting ZFPs (43ab) carrying TET2 and controls constructs (empty vector, 43ab-VP64, and TFPI2-cDNA). Pictures are a representative of two independent experiments. (b) Quantification of the colony size of CC-11 cells from a using ImageJ. Each bar represents the average colony size per condition from two independent experiments. For each condition, four pictures were randomly taken. Cell viability of HeLa (c) and CaSki (d) cells 5 days after transduction with the gene-targeting constructs measured with a MTS assay. The fraction of late apoptotic and necrotic cells 5 days after transduction with the gene-targeting zinc fingers (43ab targeting TFPI2, 5ab targeting C13ORF18, and 12ab targeting CCNA1) fused to VP64, TET2 or TET2 mutants measured with a PI assay in HeLa (e) and CaSki cells (f). Transductions were performed on superinfected cells and each data point represents the mean of three independent experiments measured in triplicate ± SEM.