Figure 3.

On-target activity of Nme and Spy CRISPR-Cas9 systems targeting endogenous loci in HEK293T cells. Both Nme and Spy gRNAs target overlapping protospacer sequences. Shown are cleavage activities of different CRISPR/Cas9 systems at eight genomic loci as measured using the T7EI assay. Data from biological replicates. For each locus both Nme Cas9 2-RNA and sgRNA gRNAs were compared to Spy Cas9. Nme 2-RNA gRNAs with significantly different values are denoted by † whereas Nme sgRNA gRNAs with significant differences in activity compared to Spy Cas9 are denoted by *. Statistical analysis was carried out for the gRNA length with the highest activity at each locus. P < 0.05.