Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Feb 16;24(3):645–654. doi: 10.1038/mt.2016.8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 Official journal of the American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

PMC Copyright notice

Cleavage activity of Spy Cas9 at on- and off-target sites. Activity of Spy gRNAs targeting (a) DHFR, (b) F8, and (c) HPRT1 at both on-target and off-target sites. (d–f) The target sequence at each locus is underlined and mismatches are highlighted in red. Sequence reads contain indels typical of non-homologous end joining repair following Cas9 cleavage. Only sites where activity levels were above 0.1% (as assessed by illumina MiSeq) are shown. Error bars represent 95% confidence intervals and P values were obtained using Fisher's exact test, *P < 0.001.