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| Reagent | Amount a (g l–1) |
| Sodium chloride (NaCl) | 7.996 |
| Sodium bicarbonate (NaHCO3) | 0.350 |
| Potassium chloride (KCl) | 0.224 |
| Potassium phosphate dibasic trihydrate (K2HPO4·3H2O) | 0.228 |
| Magnesium chloride hexahydrate (MgCl2·6H2O) | 0.305 |
| 1 M hydrochloric acid (HCl) | 40 ml |
| Calcium chloride (CaCl2) | 0.278 g |
| Sodium sulfate (Na2SO4) | 0.071 |
| Tris(hydroxymethyl) aminomethane (C4H11NO3) | 6.057 |
a Unless otherwise indicated.
