Table 2.

The technical details in detection of miR-21 expression

Study	Sample size	Sample	Sample technique	Method	Endogenous control	Cutoff
Dillhoff	80	FFPE	MDS	ISH	RNU6	Score > 1
Giovannetti a	28	Tissue	MDS	RT-PCR	RNU43	Median
Giovannetti b	31	Tissue	MDS	RT-PCR	RNU44	Median
Hwang	82	FFPE	MDS	RT-PCR	RNU66 or RNU43	Median
Jamieson	48	FT	NR	RT-PCR	RNU6	Median
Liu	38	Serum		RT-PCR	NR	NR
Nagao	65	FFPE	NR	RT-PCR	RNU6	Mean
Wang	177	Serum		RT-PCR	RNU7	Median
Kadera	145	FFPE	NR	ISH	RNU6	Median
Papaconstantinou	88	FFPE	NR	RT-PCR	RNU6	Mean
Ma	78	FT	NR	RT-PCR	RNU6	≥2-fold change
Dhayat	91	FFPE	NR	RT-PCR	NR	Mean
Khan	12	Serum		ISH	NR	Median

FFPE formalin-fixed paraffin-embedded, FT frozen tissue, MD microdissected sample, ISH in situ hybridization, qRT-PCR quantitative real-time polymerase chain reaction assay, NR not reported