Table 2.
DIGE-spots with homology with sequences in databases that were up-regulated in response to cold acclimation
| Spot no. | Homology | Functional category | CA/NA ratio |
|---|---|---|---|
| 2039 | Cold responsive protein TRVSP | Response to stress | 29.54 |
| 1648 | KS-dehydrin | Response to stress | 20.81 |
| 2051 | Cold acclimation-specific protein | Response to stress | 11.03 |
| 1776 | Actin-depolymerizing factor 2-like | Signal transduction | 9.43 |
| 1601 | Peptidyl-prolyl cis-trans isomerase 1-like | Molecular chaperones and protein folding | 5.92 |
| 1587 | Phospholipid hydroperoxide glutathione peroxidase | Response to stress (ROS scavenging) | 5.14 |
| 547 | Enolase-like | Carbohydrate and energy metabolism | 5.02 |
| 93 | Elongation factor 2-like | Transcription and translation | 4.37 |
| 353 | 2,3-bisphosphoglycerate-independent phosphoglycerate mutase | Carbohydrate and energy metabolism | 4.26 |
| 2046 | Tyrosine phosphatase | Signal transduction | 4.10 |
| 802 | Monodehydroascorbate reductase | Response to stress (ROS scavenging) | 4.07 |
| 142 | Methionine synthase | Amino acid metabolism | 4.05 |
| 1711 | Ubiquitin-conjugating enzyme | Molecular chaperones and protein folding | 3.92 |
| 318 | NADP-dependent malic enzyme | Carbohydrate and energy metabolism | 3.57 |
| 810 | 3-hydroxyisobutyryl-CoA hydrolase-like | Signal transduction (Cold stress signalling) | 3.45 |
| 1365 | Glutathione S-transferase | Response to stress (ROS scavenging) | 3.38 |
| 1286 | Mitochondrial outer membrane protein porin | Metabolite transport | 3.32 |
| 887 | Glyceraldehyde-3-phosphate dehydrogenase | Carbohydrate and energy metabolism | 3.32 |
| 1280 | Outer plastidial membrane protein porin-like | Metabolite transport | 3.29 |
| 278 | Stress-induced-phosphoprotein | Signal transduction | 3.17 |
| 760 | Transaldolase-like | Carbohydrate and energy metabolism | 3.12 |
| 598 | Elongation factor 1-gamma | Transcription and translation | 3.08 |
| 378 | Protein disulfide isomerase-like protein precursor | Molecular chaperones and protein folding | 2.98 |
| 1510 | Universal stress protein A-like protein | Response to stress | 2.95 |
| 664 | Abscisic acid stress ripening protein | Signal transduction | 2.89 |
| 1369 | Ferritin-2 | Response to stress (ROS scavenging) | 2.88 |
| 458 | Adenosylhomocysteinase-like | Amino acid metabolism | 2.87 |
| 1095 | Isoflavone reductase related protein | Response to stress (ROS scavenging) | 2.73 |
| 619 | Elongation factor 1-alpha-like | Transcription and translation | 2.65 |
| 66 | Aconitate hydratase | Carbohydrate and energy metabolism | 2.55 |
| 814 | Fructose-bisphosphate aldolase | Carbohydrate and energy metabolism | 2.40 |
| 2048 | Malate dehydrogenase | Carbohydrate and energy metabolism | 2.35 |
| 694 | Isocitrate dehydrogenase [NADP] | Carbohydrate and energy metabolism | 2.30 |
| 283 | Heat shock protein | Molecular chaperones and proteins folding | 2.20 |
| 842 | Adenosine kinase | Carbohydrate and energy metabolism | 2.18 |
| 1267 | Proteasome subunit alpha type | Molecular chaperones and protein folding | 2.00 |
| 527 | Phosphopyruvate hydratase | Carbohydrate and energy metabolism | 1.89 |
| 41 | 2-oxoglutarate dehydrogenase E1 subunit-like | Carbohydrate and energy metabolism | 1.89 |
| 792 | Glutamine synthetase | Amino acid metabolism | 1.86 |
| 939 | Quinone oxidoreductase-like protein | Response to stress (ROS scavenging) | 1.81 |
| 772 | Aspartate aminotransferase | Amino acid metabolism | 1.81 |
| 1430 | Chalcone isomerase 2 | Response to stress (ROS scavenging) | 1.80 |
| 769 | Formate dehydrogenase 1 | Carbohydrate and energy metabolism | 1.78 |
| 667 | Citrate synthase-like protein | Carbohydrate and energy metabolism | 1.74 |
| 971 | Fructokinase-2-like | Carbohydrate and energy metabolism | 1.73 |
| 580 | 6-phosphogluconate dehydrogenase, decarboxylating-like | Carbohydrate and energy metabolism | 1.70 |
For each spot, the name of the homologous sequence, the functional category and the average CA/NA abundance ratio across the six populations are listed. Spots with cold acclimated (CA) to non-acclimated (NA) abundance ratio ≥ 1.5 and with t-test, P < 0.05 in at least five populations are presented. In cases when homologous sequences were detected at different positions on the gels, the spot with the highest CA/NA ratio is presented. The exhaustive list of polypeptides up-regulated in response to cold acclimation is provided in Additional file 3: Table S4a