Table 1. Sampling localities and genetic diversity estimations for the Dactylorhiza allopolyploid populations and the diploid parental species studied here.

Acronym	Lat/Long	N	Num	Eff_num	A	P	Ho	He	FIS
D. traunsteineri s.l.
SCA5	60.623N 17.610E	12	7.25	4.21	5.82±0.40	0.24±0.12	0.71	0.75	0.050a
SCA4	57.817N 18.895E	14	8.00	4.90	6.37±0.64	0.10±0.05	0.63	0.80	0.213a
SCA3	57.34N 18.321E	20	8.12	4.14	5.34±0.77	0.06±0.03	0.70	0.72	0.020
BRI5	57.685N 7.206W	20	5.25	2.61	3.92±0.29	0.01±0.01	0.65	0.60	−0.091a
BRI4	57.767N 5.569W	5	2.75	1.96	2.75±0.37	0.00±0.00	0.56	0.48	−0.205a
BRI3	57.422N 5.819W	15	5.75	3.05	4.54±0.42	0.00±0.00	0.66	0.64	0.042
BRI2	54.282N 0.690W	9	4.50	3.02	4.15±0.52	0.13±0.13	0.65	0.65	0.007
BRI1	54.253N 0.685W	19	6.50	3.59	4.95±0.64	0.07±0.05	0.70	0.68	−0.036a
ALP8	47.341N 12.805E	34	14.12	7.19	8.12±0.81	0.11±0.04	0.81	0.83	0.033a
ALP11	47.282N 11.185E	10	3.62	2.70	3.42±0.25	0.00±0.00	0.73	0.64	−0.155a
ALP16	47.710N 15.217E	24	9.50	4.077	6.09±0.36	0.08±0.05	0.66	0.74	0.117a
Mean±s.e.			6.85±0.95	3.77±0.43	5.04±0.46	0.07±0.02	0.68±0.02	0.68±0.03	0.000±0.03
D. majalis s.s.
SCA2	55.931N 14.068E	11	7.50	4.61	6.44±0.36	0.18±0.10	0.84	0.79	−0.064a
SCA1	55.818N 12.946E	17	7.25	4.35	5.56±0.67	0.02±0.02	0.78	0.74	−0.067a
PYR3	42.859N 0.495E	14	8.37	4.66	6.15±0.91	0.24±0.12	0.80	0.75	−0.066a
PYR1	42.862N 1.9810E	18	12.25	6.94	8.33±0.64	0.48±0.24	0.80	0.85	0.062
ALP1	47.679N 15.646E	10	8.62	5.11	7.00±0.57	0.02±0.01	0.85	0.81	−0.064a
ALP4	47.586N 15.100E	9	5.50	3.66	5.02±0.32	0.09±0.07	0.78	0.74	−0.056a
ALP7	47.795N 13.393E	6	8.62	5.47	8.01±0.72	0.03±0.03	0.79	0.82	0.044a
ALP10	47.745N 15.351E	17	11.50	6.54	7.85±0.86	0.11±0.04	0.85	0.83	−0.023
ALP12	47.319N 11.542E	9	9.37	5.93	7.75 ±0.82	0.16±0.10	0.82	0.82	−0.009
ALP14	47.282N 12.774E	17	11.50	6.92	7.98±0.78	0.14±0.07	0.79	0.84	0.059a
Mean±s.e.			9.05±0.68	5.42±0.36	7.01±0.36	0.15±0.04	0.81±0.01	0.80±0.01	−0.018±0.017
Locally sympatric
ALP9	47.461N 12.366E	24	14.00	7.29	8.27±0.92	0.20±0.07	0.84	0.82	−0.023a
ALP13	47.529N 12.579E	30	14.87	7.46	8.20±0.97	0.31±0.17	0.72	0.82	0.124a
ALP15	47.814N 13.244E	22	11.50	6.02	7.48±0.74	0.06±0.03	0.82	0.81	−0.015
Mean±s.e.			13.4±1.01	6.92±0.45	7.95±0.25	0.19±0.07	0.79±0.04	0.82±0.01	0.028±0.05
Diploids
D. fuchsii	Supplementary Table 1	19	8.25	5.92	6.88±1.14	0.77±0.32	0.61	0.78	NA
D. incarnata	Supplementary Table 1	23	7.12	4.56	5.86±0.95	0.27±0.14	0.30	0.70	NA

Acronyms correspond to the localities given in Figure 1. Abbreviations: N, number of individuals studies per population; Num, average number of alleles per locus; Eff_num, the effective number of alleles; A, allelic richness; P, private alleles; Ho, observed heterozygosity; He, expected heterozygosity, F_IS, inbreeding coefficient.

^aIndicate values significantly different from 0. NA indicates not applicable.