Table 2. Primer sequences and characteristics of eight microsatellite loci newly reported here for Dactylorhiza.
| Locus | Primer sequence | Observed size range (bp) | PIC2x | HO2x | NA2x | NA4x | Genbank Acc. (D.f./D.i.) | Repeat motif (D.f./D.i.) |
|---|---|---|---|---|---|---|---|---|
| D2 | F-CAGTCGGGCGTCATCATGAAATACACGAAGGGCGAG R-GTTTGATCGCCGGACTTAACCG | 291–319 | 0.78 | 0.81 | 7 | 14 | KJ209801/KJ209802 | (GA)6/(GA)11 |
| D5 | F-CAGTCGGGCGTCATCATTTGACGAAGTCAGGCATCTC R-GTTTCGCTAGAAAGCTCCGTTGAC | 134–172 | 0.88 | 0.89 | 13 | 20 | KJ209803/KJ209804 | (CT)9(TC)7/(CT)10(TC)9 |
| D8 | F-CAGTCGGGCGTCATCAATTCCACTCCGAGCAAAC R-GTTTGCGACGWGTGACTTTATCCA | 309–361 | 0.89 | 0.9 | 16 | 25 | KJ209806/KJ209805 | (TC)8/(TC)18 |
| D16 | F-CAGTCGGGCGTCATCATTGCTGTTTGCTGGGAGAC R-GTTTCTCYTCTACCCTCCAACCC | 208–286 | 0.94 | 0.94 | 29 | 38 | KJ209808/KJ209807 | (TC)14/(TC)7TT(TC)23 |
| D51 | F-CAGTCGGGCGTCATCAAAGTAATGGGCTTTATGCTATTTGA R-GTTTATTGGAATACATTACAAGGATGTTCA | 90–124 | 0.65 | 0.67 | 12 | 18 | KJ209809/KJ209810 | (AG)14/(AG)8 |
| D52 | F-CAGTCGGGCGTCATCACCTCCTCTCCCTCCTCTTGT R-GTTTGGGCACTACTACACCTGTCTCAA | 172–228 | 0.69 | 0.71 | 7 | 24 | KJ209812/KJ209811 | (CT)7…(CT)4/(CT)9…(CT)14 |
| D55 | F-CAGTCGGGCGTCATCATCCGTGAGTGGATTTCGTCT R-GTTTAGGAAGCTCCGCTCTCTCAG | 296–342 | 0.79 | 0.81 | 11 | 19 | KJ209814/KJ209813 | (CG)3(AG)4/(CG)6(AG)13 |
| D81 | F-CAGTCGGGCGTCATCATTTCGAAACATTTCTCCTCCG R-GTTTGCGTAACCCTAGCGTTGTCT | 84–116 | 0.87 | 0.88 | 15 | 17 | KJ209815/KJ209816 | (CT)14/(CT)20 |
Abbreviations: PIC2x, polymorphism information content; HO2x, observed heterozygosity; NA2x and NA4x, total number of alleles observed within diploids and allotetraploids, respectively. Genbank accession numbers and repeat motives are reported separately for the references D. fuchsii and D. incarnata, respectively. The parts of the primer sequence that contain the tails introduced for multiplexing are shown with bold letters.