Table 2.

Oligonucleotides used for mutant and complement strains construction in this work

Primer	Sequence
Amplification of the Cm cassette
Forward	GAATTTTTTCGCTATAGTGTAGGCTGGAGCTGCTTC
Reverse	GAATTTTTTCGCTATACATATGAATATCCTCCTTAG
Mutagenesis
Forward	TATTCTTTAGGGCTATGGTTTTTCATTTTTTACCGGAAGTTACCGACGTTGTGTAGGCTGGAGCTGCTTC
Reverse	AGTCTCGCGTGGAAGCTGCGGTATGGGTGCATCAGGAGCGCATTTTCTGACATATGAATATCCTCCTTAG
Mutagenesis confirmation
Forward	TACCGGAAGTTACCGACGTT
Reverse	ATCAGGAGCGCATTTTCTGA
Genetic Complementation
Forward	GCTATAGTCGACGCGAGAAAATACGACAAAAG
Reverse	GTAAGGATCCCACATGCTGAAGAAAATGAA