Figure 2. Effect of LPS on [Ca²⁺]i.

(A) Effect of LPS (1 µg/ml) on [Ca²⁺]i before and after extracellular Ca²⁺ removal by EGTA (1 mM) in representative HLMs. Each trace is representative of 20 cells recorded in 3 different experiments. (B) Quantification of the effect exerted by LPS on [Ca²⁺]i in absence or presence of extracellular Ca²⁺. *P < 0.05 vs. untreated, **P < 0.05 vs. control. (C) Effect of LPS on 2-AG production after intracellular and extracellular [Ca²⁺] removal by BAPTA (10 µM) and EGTA (1 mM), respectively. HLMs were pretreated for 5 min with BAPTA and then for 15 min with EGTA before stimulation with LPS (15 min). Data are mean ± sd of 4 independent experiments with cells from different donors. *P < 0.05 vs. respective control; **P < 0.05 vs. LPS alone.