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. 2015 Oct 17;43(20):9694–9710. doi: 10.1093/nar/gkv1063

Figure 4.

Figure 4.

The histone demethylase JMJD-1.2 regulates H3K23me2 levels in the oocytes. (A) Extracted gonads from wild type (top) and jmjd-1.2(tm3713) (bottom) were fixed and stained with H3K23me2 antibody (right). DNA was counterstained with DAPI (left). Arrowheads indicate some oocytes nuclei at the diakinesis stage. (B) Magnification of oocytes from extracted gonads of wild type and jmjd-1.2(tm3713) adult hermaphrodite fixed and stained with H3K23me2 antibody (middle). DNA was counterstained with DAPI (left). Overlay (right). Scale bar is 5 μm. (C) Demethylation assay on C. elegans histones incubated with recombinant JMJD-1.2 (30 μg). The reaction was probed with the indicated antibodies. Ponceau staining of purified JMJD-1.2 used for the reaction is presented at the bottom.