Figure 7.

HPL-1 recruits a CoREST-like complex. (A) List of the HPL-1::GFP interactors, identified by MS. Gene name, description, domains, human homolog,% of sequence coverage, number of unique peptides and total number of peptides identified are shown. (B) HPL-1::GFP interacts with endogenous SPR-5. Total protein extracts (2 mg) from embryos of the indicated transgenic animals (hpl-1::GFP and hpl-2::GFP) were immunoprecipitated using anti-GFP antibody. The precipitates were analyzed by SDS-PAGE followed by western blotting with antibodies against GFP and SPR-5. Input = 50 μg of protein extract, Control = empty beads. (C) Proposed model for HPL-1 recruitment in heterochromatic foci in C. elegans embryos and its binding partners.