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(A) Hygromycin A—chloramphenicol competition for binding to the 50S ribosomal subunit. Release of 50S ribosomes-bound [14C]-chloramphenicol upon addition of increasing concentrations of HygA. Further details are given in ‘Materials and Methods’ section. (B) Comparison of the inhibitory activities of HygA (blue) and chloramphenicol (black) on 027 mRNA translation in a cell free extract of a Thermus thermophilus mutant (23S rRNA G2061A; (47)).
