Table 1. Data Collection and refinement Statistics.
| Complex | 50S apo | 50S–HygA |
|---|---|---|
| PDB accession code | 5DM6 | 5DM7 |
| Data collection statistics | ||
| Space group | I222 | I222 |
| Cell dimensions, Å (a, b, c) | 169.90, 410.76, 696.12 | 169.82, 411.54, 695.65 |
| Resolution range, Å | 59.3–2.90 (3.06–2.90) | 59.3–3.00 (3.16–3.00) |
| Resolution where I/σI = 2, Å | 3.2 | 3.7 |
| No. of observed reflections | 11 664 835 (1 754 909) | 2 197 039 (315 299) |
| No. of unique reflections | 531 662 (78 466) | 479 454 (68 449) |
| Multiplicity | 21.9 (22.3) | 4.58 (4.60) |
| Completeness,% | 99.8 (99.6) | 99.5 (99.1) |
| Rmerge,% | 24.1 (786.2) | 21.5 (562.3) |
| I/σI | 10.38 (0.71) | 6.28 (0.33) |
| CC(1/2),% | 99.9 (47.8) | 99.8 (18.5) |
| Wilson B-factor, Å2 | 78.94 | 66.56 |
| Refinement statistics | ||
| Rwork/Rfree,% | 24.1/28.2 | 26.5/31.5 |
| No. of atoms | ||
| RNA | 62 274 | 62 274 |
| Protein | 27 162 | 27 162 |
| Ligand | 0 | 36 |
| Ions | 198 | 197 |
| RMSD | ||
| Bond lengths, Å | 0.014 | 0.027 |
| Bond angles, ° | 2.323 | 2.407 |
Values in parentheses refer to the highest resolution bin.
Each dataset was obtained from a single crystal.