Table 1. Steady-state kinetic constants for ATP-[32P]PPi exchange by T1 and T2 enzymes.
Thr | Ser | ||||||
---|---|---|---|---|---|---|---|
KM (mM) | kcat (s−1) | kcat/KM (mM−1 s−1) | KM (mM) | kcat (s−1) | kcat/KM (mM−1 s−1) | Specificity factor (kcat/KM)Thr/(kcat/KM)Ser | |
T1 | 2.6 ± 0.3 | 10.0 ± 1.5 | 3.8 ± 0.7 | 274 ± 4 | 3.290 ± 0.014 | 0.0120 ± 0.0002 | 314 |
T2 | 0.22 ± 0.03 | 4.42 ± 0.13 | 20.1 ± 2.6 | 77 ± 14 | 3.6 ± 0.4 | 0.046 ± 0.010 | 436 |
Numbers indicate average ± standard deviation (n = 3).