Table 2. Data obtained from measurements at 50 nm/s (a) or theoretical prediction (b).

	(a)	(a)	(b)	(b)	(b)
Hairpin	Transition	Transition	ΔG	ΔG	ΔG
	force Ft	length L	mfold	stretch	total
	(pN)	(nm)	(kJ/mol)	(kJ/mol)	(kJ/mol)
DNA10	6.3	14.8 ± 2	63± 3	20 ± 1	83 ± 4
DNA18	4.85	16.6 ± 2	60 ± 3	18 ± 1	78 ± 4
RNA10	11.2	17.7 ± 2	89 ± 4	32 ± 2	121 ± 6
RNA18	9.95	18.4 ± 2	87 ± 4	35 ± 2	122 ± 6

mfold: http://mfold.rna.albany.edu, see Materials and Methods and Supplementary Information for parameter determination.