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Experiment showing detection limits on native PAGE gels. Oligonucleotide sequences HP-18 (left panels) and BCL-2 (right panels) were tested at nine concentrations (0.125, 0.25, 0.5, 0.7, 1, 2, 2.5, 3 and 5 μM). RNA samples were prepared in 20 mM Tris-HCl buffer with 40 mM K+ and loaded on a non-denaturing 12% acrylamide gel. The 12% acrylamide gels (12%) were stained with either (A) 20 μM CyT or (B) SYBR Gold, and were visualized on GE Typhoon Trio.
