Figure 2.

Role of the Spt5 CTD in formation of H3K36me3. (A) Whole-cell extracts derived from the indicated strains (top) were subjected to SDS-PAGE and immunoblotting with the indicated antibodies (right). ‘WT’ (wild-type) is described in Materials and Methods. The spt5-CTD-7 strain harbors a truncated form of the CTD containing 7 consensus nonapeptide repeats that is functional for growth at all temperatures. The mutant strains harbor similar CTDs bearing the indicated mutations in all of the repeats or lack the CTD entirely (spt5ΔCTD). (B) As in (A). (C) As in (A) and Figure 1. Two exposures of the same H3K36me3 blot are shown.