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. 2015 Aug 14;43(20):9766–9775. doi: 10.1093/nar/gkv837

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — The role of Mcs6 activity in co-transcriptional histone modifications. (A) Strains carrying the indicated analogue sensitive (as) alleles were treated with DMSO (−) or 40 μM 3-MB-PP1 (+) for 3 h prior to extract preparation. Whole-cell extracts derived from each sample were analyzed by SDS-PAGE and immunoblotting with the indicated antibodies (right). (B) As in (A) for strains harboring mcs6⁺ or mcs6^as in combination with the rpb1-CTD-S5-MCE1 allele. Dashes indicate the position of the 250 kD size marker. (C) As in (A) for strains harboring the indicated mcs6 and rpb1 alleles.