Figure 4.

Co-targeting also works in U2OS cells. (A) Staining of colonies formed after U2OS cells were transfected with different plasmids and selected with 10μg/ml 6-TG. (B) Sequences of PCR products of the AAVS1 region in the AAVS1 targeted but unselected cells, 6-TG resistant HPRT and AAVS1 co-targeted cells or untargeted U2OS cells. (C) Sequences of individual inserts of the cloned PCR products from the genomic DNA of the co-targeted 6-TG resistant cells. (D) Sequencing chromatogram of the most frequent mutation.