Figure 5.

Co-targeting allows quick evaluation of gene essentiality and guide RNA effectiveness. (A) Staining of colonies formed after HCT116 cells were transfected with different plasmids and selected with 10μg/ml 6-TG. Cells were seeded at 0.05% (no 6-TG) and 5% (+6-TG). The HCT116 cells in these experiments carried an integrated Cas9 gene under the control of the TET promoter. (B) Sequencing chromatogram of the BLM region of the genomic DNA isolated from the pooled HPRT and BLM co-targeted 6-TG resistant cells.