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. 2015 Aug 18;43(17):8435–8451. doi: 10.1093/nar/gkv844

Figure 3.

Figure 3.

SRSF11 associates with catalytically active telomerase in a cell cycle-dependent manner. (A) SRSF11 associates with active telomerase in a cell cycle-dependent manner. HeLa S3 cells were synchronized by double thymidine block and harvested at 2 h intervals over a 10 h time course after release. The 0 h time point corresponds to blocked cells before release. Immunoprecipitates of endogenous SRSF11 from synchronized cells were subjected to immunoblotting and quantitative RT-PCR as indicated. Immunoblot for proliferation cell nuclear antigen (PCNA) was used for an independent marker of S phase. Band intensities were quantified and displayed as fold change in the accompanying graph. AS, asynchronous cells. (B) Immunoprecipitates of endogenous SRSF11 from synchronized cells were analyzed for telomerase activity by the TRAP assay. IgG was used as a negative control. Telomerase products were quantified and displayed as fold change in the accompanying graph. ITAS, internal telomerase assay standard.