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. 2015 Aug 18;43(17):8435–8451. doi: 10.1093/nar/gkv844

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — SRSF11 is required for telomere elongation. (A) HeLa S3 cells stably expressing control shRNA (shControl) or SRSF11 shRNAs (shSRSF11–1 and shSRSF11–2) were harvested at the indicated population doublings (PDs) and subjected to immunoblotting to measure the levels of various telomerase components. (B) HeLa S3 cells stably expressing shControl or shSRSF11 were harvested at PD 99 and subjected to immunoblotting to measure the levels of various shelterin proteins. (C) HeLa S3 cells stably expressing shControl or shSRSF11 were harvested at the indicated PDs and analyzed for telomerase activity by the TRAP assay. ITAS, internal telomerase assay standard. (D) HeLa S3 cells stably expressing shControl or shSRSF11 were harvested at the indicated PDs, and genomic DNA was digested with Hinf1 and Rsa1, followed by Southern blot analysis using a telomere repeat probe. (E) Graphical representation of average telomere length versus population doubling number.