Figure 6.

NMR analysis of full-length 3′X domain of HCV. (A) ¹H–¹⁵N HSQC spectrum of X98 at low ionic strength (green), superposed with those of the SL1 (blue) and SL2’ (red) constructs acquired under the same temperature and ionic conditions. The assignments appear in blue and red for crosspeaks of nt belonging to subdomains SL1’ and SL2’, respectively. The SL1 G53 and U55 HN crosspeaks that do not match 3′X signals are marked with black assignments. Conditions: 34 μM X98, 0 mM NaCl/MgCl₂, 27°C. (B) Secondary structure of the 3′X domain monomer supported by the NMR data.