Table 1. Number of GUS/SUG siRNA reads found in different backgrounds.
6b4/SUG6 | L1 | |||
---|---|---|---|---|
RPM | Ratio to WT | RPM | Ratio to WT | |
WT | 15 605 | 1.00 | 48 865 | 1.00 |
dcl2 | 8251 | 0.53 | 23 525 | 0.48 |
dcl4 | 9630 | 0.62 | 57 253 | 1.17 |
dcl2dcl4 | 528 | 0.03 | 511 | 0.01 |
ago1–27 | 4523 | 0.29 | 426 | 0.01 |
ago1-1 | 3808 | 0.24 | 474 | 0.01 |
rdr6 | 4120 | 0.26 | 514 | 0.01 |
20 to 25 nucleotides long reads aligning to transgene sequences are reported in read per million (RPM). The ratio to the WT background sample of the different series (6b4/SUG6 and L1) is given on the right.