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Domains of FMRP interacting with the mRNA of GRK4. (A) Architectural organization of FMRP domains. (B) Filter binding assay, using various recombinant RNA-binding domains of FMRP: KH1, KH2 and the C-terminal domain containing the RGG box and 32P-labeled RNAs, reveals that the FMRP C-terminal domain (FCT) displays equal affinity for Cod2 fragment or G-quadruplex (N19 RNA), whereas the two KH domains are not able to bind Cod2 mRNA as well as N19 RNA.
