Figure 4.

Sequences of the GRK4 mRNA interacting with FMRP. (A) Scheme of the different subclones we generated from Cod2. Their size and their position in the coding sequence of GRK4 are indicated. (B) Filter binding assay using FMRP and ³²P-labeled N19. The competition was performed using various regions of unlabeled GRK4 mRNA: Cod2a and Cod2b fragments. The two control N19 and Δ35 were also used as competitors. (C) Filter binding assay using FMRP and ³²P-labeled N19. The competition was performed using various regions of unlabeled GRK4 mRNA: Cod2a-α and Cod2a-β fragments. The two control N19 and Δ35 were also used as competitors. Each experiment was repeated four times.