Table 2. Primers used for cloning into psiCHECK-2 vector.
Fragments | Primers |
---|---|
S1/S2 | F:TGCTTGGTGCTCACCATTATGAATGGAGGGGATTTGAAGTTTCACATTT |
R:GGCTCTCTGCTCATCAAAGCCGGGATTGCCCAGGTTGTAAATGTGAAA | |
DegS1/S2 | F:TGCCTGGTGCTGACCATTATGAACGGCGGCGATCTGAAATTTCATATTT |
R:CGCGCGCTGTTCATCAAAGCCCGGGTTGCCCAGGTTATAAATATGAAAT | |
S3/S4 | F:TTTGATGAGCAGAGAGCCGTTTTCTATGCTGCAGAGCTGTGTTGCGGC |
R: GTATACAATTCTTTCCCTCTGTAAATCTTCCAAGCCGCAACACAGCTCT | |
DegS3/S4 | F: TTTGATGAACAGCGCGCGGTGTTTTATGCGGCGGAACTGTGCTGCGGC |
R:ATACACAATGCGTTCGCGCTGCAGATCTTCCAGGCCGCAGCACAGTTCC |