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. 2015 Aug 6;43(17):8540–8550. doi: 10.1093/nar/gkv801

Table 2. Primers used for cloning into psiCHECK-2 vector.

Fragments Primers
S1/S2 F:TGCTTGGTGCTCACCATTATGAATGGAGGGGATTTGAAGTTTCACATTT
R:GGCTCTCTGCTCATCAAAGCCGGGATTGCCCAGGTTGTAAATGTGAAA
DegS1/S2 F:TGCCTGGTGCTGACCATTATGAACGGCGGCGATCTGAAATTTCATATTT
R:CGCGCGCTGTTCATCAAAGCCCGGGTTGCCCAGGTTATAAATATGAAAT
S3/S4 F:TTTGATGAGCAGAGAGCCGTTTTCTATGCTGCAGAGCTGTGTTGCGGC
R: GTATACAATTCTTTCCCTCTGTAAATCTTCCAAGCCGCAACACAGCTCT
DegS3/S4 F: TTTGATGAACAGCGCGCGGTGTTTTATGCGGCGGAACTGTGCTGCGGC
R:ATACACAATGCGTTCGCGCTGCAGATCTTCCAGGCCGCAGCACAGTTCC