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In vitro insertion and extension assay of the dG3-C8-IQ adduct by ypol ζ. (A) Insertion of dCTP opposite a control unmodified dG (left) and reaction of the dG-C8-IQ modified oligonucleotide in the presence of all four dNTPs. (B) Extension of the dG3-C8-IQ adduct when paired with C or A after 5 (left) and 24 (right) h.
