Figure 6.

mucR encodes a DGC (A) The expression of cdrA-lux was induced in the wild-type PAO1 strain carrying multiple copies of a plasmid expressing mucR or mucR_DGC. (B) The overexpression of mucR or mucR_DGC increased the intracellular level of c-di-GMP. LC-MS/MS measurement of c-di-GMP revealed that the PAO1 strain containing p-mucR or p-mucR_DGC accumulates significantly more intracellular c-di-GMP than the PAO1 strain containing the empty vector. Data are shown as mean ± SEM from three experiments. Statistical significance was determined using Student's t-test (***P < 0.001). EV represents empty vector.