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Schematic representation of the LibSeq strategy. LibSeq experiments are performed with a pool of reporter plasmids that are identical except for a seven nucleotide sequence located in the 3′ UTR of the reporter gene. Each individual 7mer sequence can affect the expression of the reporter mRNA through interactions with endogenous or exogenous regulator molecules and at the same time serve as a barcode allowing the expression to be evaluated using massive parallel sequencing.
