Table 1. The melting characteristics of the studied DNA duplexes at 283 μM concentration in 150 mM NaCl, 5 mM Na-Phosphate pH 7.4.
| DNA duplexes | Tt (°C) |
(kJ/mol) |
(kJ/mol) |
|
|---|---|---|---|---|
| CG duplexes | ||||
| 9-CG | 5′-CGCCGCCGC-3′ | 74.0 | 290 | 223 |
| 3′-GCGGCGGCG-5′ | ||||
| 12-CG | 5′-CGCCGCCGCCGC-3′ | 83.6 | 420 | 323 |
| 3′-GCGGCGGCGGCG-5′ | ||||
| 15-CG | 5′-CGCCGCCGCCGCCGC-3′ | 89.5 | 530 | 408 |
| 3′-GCGGCGGCGGCGGCG-5′ | ||||
| AT duplexes | ||||
| 9-AT | 5′-CGCAAACGC-3′ | 60.4 | 285 | 251 |
| 3′-GCGTTTGCG-5′ | ||||
| 12-AT/AT | 5′-CGCAAAAAACGC-3′ | 63.0 | 410 | 360 |
| 3′-GCGTTTTTTGCG-5′ | ||||
| 12-AT/TA | 5′-CGCAAATTTCGC-3′ | 64.5 | 405 | 350 |
| 3′-GCGTTTAAAGCG-5′ | ||||
| 12-TA/AT | 5′-CGCTTTAAACGC-3′ | 60.8 | 371 | 327 |
| 3′-GCGAAATTTGCG-5′ | ||||
| 12-A/T | 5′-CGCATATATCGC-3′ | 60.3 | 370 | 326 |
| 3′-GCGTATATAGCG-5′ | ||||
| 15-AT/TA/AT | 5′-CGCAAATTTAAACGC-3′ | 64.8 | 500 | 440 |
| 3′-GCGTTTAAATTTGCG-5′ | ||||
| 15-AT/AT/AT | 5′-CGCAAAAAAAAACGC-3′ | 65.1 | 503 | 443 |
| 3′-GCGTTTTTTTTTGCG-5′ | ||||
| Errors | ±0.3 | ±15 | ±10 | |

