Table 3. The DSC-measured total and cooperative enthalpy, entropy and Gibbs energy of association of CG and AT base pairs, extrapolated to the standard temperature of 25°C.

CG duplexes	−ΔHCG (kJ/mol-bp)		−ΔSCG (J/K·mol-bp)		−ΔGCG (J/K·mol-bp)
	coop	total	coop	total	coop
9-CG
5′-CGCCGCCGC-3′	18.4	25.8	39.7	62.5	6.6
3′-GCGGCGGCG-5′
12-CG
5′-CGCCGCCGCCGC-3′	19.2	27.4	40.4	63.4	6.3
3′-GCGGCGGCGGCG-5′
15-CG
5′-CGCCGCCGCCGCCGC-3′	18.7	26.3	41.8	66.1	6.2
3′-GCGGCGGCGGCGGCG-5′
Averaged	18.8±0.5	26.5±1.0	40.6±1.0	64.0±1.5	6.4±0.2
AT duplexes	−ΔHAT		−ΔSAT		−ΔGAT
	coop	total	coop	total	coop
9-AT
5′-CGCAAACGC-3′	31.5	35.3	86	98	5.8
3′-GCGTTTCGG-5′
12-AT/AT
5′-CGCAAAAAACGC-3′	30.1	34.3	86	98	4.5
3′-GCGTTTTTTGCG-5′
12-AT/TA
5′-CGCAAATTTCGC-3′	29.0	32.5	82	93	4.6
3′-GCGTTTAAAGCG-5′
12-TA/AT
5′-CGCTTTAAACGC-3′	25.1	28.8	70	80	4.2
3′-GCGAAATTTGCG-5′
12-A/T
5′-CGCATATATCGC-3′	25.1	28.8	68	80	4.8
3′-GCGTATATAGCG-5′
15-AT/TA/AT
5′-CGCAAATTTAAACGC-3′	27.8	32.4	78	93	4.5
3′-GCGTTTAAATTTGCG-5′
15-AT/AT/AT
5′-CGCAAAAAAAAACGC-3′	28.1	32.0	79	91	4.5
3′-GCGTTTTTTTTTGCG-5′
Averaged	28.1±1.8	32.0±2.0	78±5.0	90±6.0	4.7±0.3