Figure 6.
Growth curve analysis of M. smegmatis wild type (WT) and ΔrecA strains harboring different plasmids in the absence and presence of 2 mM H2O2 or 1 mM NaNO2.M. smegmatis strains (3–5 replicates) were used for growth curve analysis in 100 well microtiter plates. Aliquots (200 μL) of 10−2 dilutions of saturated cultures in LB-Tween supplemented with Hyg and 0.5% (w/v) BSA were taken in the absence or presence of H2O2 or NaNO2. For growth in NaNO2, the medium was adjusted to a pH of 5.5. Growth was monitored as OD600 at 37°C for 50 h using Bioscreen C kinetic growth reader under constant shaking. Growth curves were prepared from the growth of three independent colonies for each strain and the mean ±SD were plotted.