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. 2016 Jan 28;25(7):1392–1405. doi: 10.1093/hmg/ddw021

Figure 7.

Figure 7.

Ub-SMN(K0) localizes in the cytoplasm and the nucleolus, and expressing Ub-SMN(K0) impairs canonical CBs and coilin/snRNP co-localization. (A) Co-immunostaining of FLAG and coilin in SMN knockdown SH-SY5Y cells stably expressing FLAG-Ub-SMN(K0) or FLAG-SUMO1-SMN(K0). Arrow denotes potential nucleoli. (B) Co-immunostaining of fibrillarin and FLAG in SMN knockdown SH-SY5Y cells stably expressing FLAG-SMN(K0) or FLAG-Ub-SMN(K0). Arrows denote areas of co-localization of fibrillarian and FLAG. (C) Co-immunostaining of coilin and FLAG in SMN knockdown SH-SY5Y cells stably expressing FLAG-Ub-SMN(K0). Arrows denote areas of co-localization of coilin and FLAG. (D) Quantification of canonical CBs (>0.3 µm in diameter) in SMN knockdown SH-SY5Y cells stably expressing FLAG-SMN or FLAG-Ub-SMN(K0). Three different areas of each staining were chosen for CB counting (50 cells/area). Error bars represent SD. (E) Co-immunostaining of SmB/B′ and coilin in SMN knockdown SH-SY5Y cells stably expressing FLAG-Ub-SMN(K0). The red arrow denotes an area of co-localization of coilin and SmB/B′; white arrows denote CBs that did not have Sm co-localization.