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. 2016 Mar 8;57(3):1038–1051. doi: 10.1167/iovs.15-17758

Figure 5.

Figure 5

Overexpression of PGC-1α does not alter hfRPE signature gene expression and functions. (A) Gene expression analysis by qPCR of ARPE-19 and hfRPE infected with Ad-GFP or Ad-mPGC-1α (n = 3 per group). (B) Western blot of RPE proteins in hfRPE infected with Ad-GFP or Ad-mPGC-1α. (C) Quantification of Western blots (n = 4 per group). (D) Western blot of rhodopsin following outer segment phagocytosis in hfRPE 0, 4, and 12 hours after phagocytosis. (E) Quantification of Western blot (n = 3 per time point). (F) Transepithelial resistance of hfRPE pre- and postinfection with Ad-GFP and Ad-mPGC-1α (n = 26 per group, paired t-test, Ad-GFP P < 0.0001; Ad-PGC-1α P = 0.1600).