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. Author manuscript; available in PMC: 2016 Mar 11.
Published in final edited form as: Stem Cells. 2015 Oct;33(10):3065–3076. doi: 10.1002/stem.2090

Figure 3.

Figure 3

Osteogenesis resulting from increased nuclear actin requires Runx2 activity. (A): Mouse marrow-derived mesenchymal stem cell (mdMSC), ± CytoD for 3 days at 24 hours after Runx2 siRNA treatment; a, b ≠ control and differ from each other, p < 0.01. (B, C): Nuclear and cytoplasmic IB, 3-day treatment of cytochalasin D after importin 9 knock-down. Densitometry of nuclear Yes-associated protein (YAP) is shown in (B, C), n = 3, *, p < 0.05. (D): Reverse transcriptase polymerase chain reaction (RT-PCR) analysis ± leptomycin B (5 ng/ml) and ± CytoD for 3 days; a, b ≠ control and differ from each other with p < 0.01. (E): mdMSC transfected with YAP construct or empty vector. IB, left panel. RT-PCR ± CytoD for 3 days. a, b ≠ control and differ from each other, p <0.01. (F): mdMSC treated with CytoD for 0 (3−), 3d (3+), or on the first day only (1+/2−). IB for nuclear YAP. Densitometry of nuclear YAP bands is shown, n = 3, *, p< 0.05. (G): 2D and 3D images. mdMSC ± CytoD for 3 days, phalloidin stain. Scale bars = 25 μm. Abbreviations: CTL, control; CytoD, cytochalasin D; LDH, lactate dehydrogenase; PARP, polyribose polymerase; YAP, Yes-associated protein.