Table 1. Knockdown of HClpP partially restores cisplatin sensitivity to KB CP20 cells.

KB CP20 cells were treated with si-HCLPP or control si-RNA for 8 h, placed in fresh medium for 16 h, after which they were incubated with 10 or 20 µg/ml cisplatin for 36 h. Apoptotic cell death was measured by flow cytometry of Annexin V-stained cells. Averages of the mean of the distribution of stained cells and the percentage of stained cells (apoptotic or dead) are reported. Significance was evaluated in Excel using the TTEST; siC vs. siP at 10 and 20 µg/ml CP): P <0.01; mean and % dead cells for 0 vs. 20 µg/ml CP): P <0.01.

	Annexin V staining
Cisplatin Treatment*	si-Control		si-HCLPP
(µg/ml)	Mean staining	% dead cells	Mean staining	% dead cells
0	30 ± 2	11 ± 1	30 ± 2	12 ± 1
10	32 ± 2	15 ± 2	62 ± 4	26 ± 2
20	37 ± 3	17 ± 2	61 ± 4	28 ± 3

^*Cisplatin-treated cultures were harvested at 36 h. Cells without cisplatin were taken at the time of cisplatin addition to the other cultures.