Figure 2.

Mates of males knocked down for each of nine secondary-cell-expressed genes have an increased remating rate. The percentage of previously mated females that were willing to remate 4 days ASM to a wild-type male within 1 hr of exposure. At 4 days ASM females initially mated to males knocked down for CG14069, CG14292, CG15406, CG15902, CG3285, CG3349, CG43161, CG7882, and CG9509 are significantly more receptive to courting males compared to mates of control males (Wilcoxon ranked-sums test: *P < 0.05, **P < 0.01, and ***P < 0.0001; control—n = 14–42, average 25; RNAi—n = 14–39, average 25; specific values can be found in Table S2). Knockdown of CG31326, the potential off-target for CG15406, did not show a phenotype, confirming that the phenotype seen with knockdown of CG15406 is not the consequence of off-target effects. These results were confirmed using additional lines for all significant genes where available (Figure S3).